› 2o27

X-ray diffraction
2.2Å resolution

Structure of a class III RTK signaling assembly

Released:
DOI: 10.2210/pdb2o27/pdb
PDB entry 2o27 coloured by chain, front view.
PDB entry 2o27 coloured by chain, side view.
PDB entry 2o27 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for stem cell factor-KIT signaling and activation of class III receptor tyrosine kinases.
Liu H, Chen X, Focia PJ, He X
EMBO J 26 891-901 (2007)
PMID: 17255936

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble KIT ligand Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P20826 (Residues: 28-166; Coverage: 56%)
Gene names: Kitl, Kitlg, Mgf, Sl, Slf
Sequence domains: Stem cell factor
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P65
Unit cell:
a: 43.27Å b: 43.27Å c: 248.433Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.242 0.266
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

Cell signaling by receptor tyrosine kinases.
Lemmon et al. (2010)
13 more

1 mention without citation

Structural basis for stem cell factor-KIT signaling and activation of class III receptor tyrosine kinases.
Liu et al. (2007)