2o2s

X-ray diffraction
2.6Å resolution

The structure of T. gondii enoyl acyl carrier protein reductase in complex with NAD and triclosan

Released:

Function and Biology Details

Reaction catalysed:
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-180409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-acyl carrier reductase ENR Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 33.39 KDa
Source organism: Toxoplasma gondii RH
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6UCJ9 (Residues: 103-417; Coverage: 81%)
Gene names: ENR, TGRH88_064800
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3221
Unit cell:
a: 78.075Å b: 78.075Å c: 187.955Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.205 0.267
Expression system: Escherichia coli