› 2o3k

X-ray diffraction
2.3Å resolution

Yeast Cytosine Deaminase D92E Triple Mutant bound to transition state analogue HPY

Released:
DOI: 10.2210/pdb2o3k/pdb
PDB entry 2o3k coloured by chain, front view.
PDB entry 2o3k coloured by chain, side view.
PDB entry 2o3k coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Entry authors: Korkegian AM, Stoddard BL

Function and Biology Details

Reaction catalysed: 
Cytosine + H(2)O = uracil + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171310 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosine deaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 161 amino acids
Theoretical weight: 17.83 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12178 (Residues: 1-158; Coverage: 100%)
Gene names: FCY1, YP9499.17, YPR062W
Sequence domains: Cytidine and deoxycytidylate deaminase zinc-binding region
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand HPY 2 x HPY
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 55.916Å b: 69.09Å c: 74.174Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.257
Expression system: Escherichia coli

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