› 2o69

X-ray diffraction
2Å resolution

Crystal Structure of Haemophilus influenzae N193L mutant FbpA

Released:
DOI: 10.2210/pdb2o69/pdb
PDB entry 2o69 coloured by chain, front view.
PDB entry 2o69 coloured by chain, side view.
PDB entry 2o69 coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
The role of the synergistic phosphate anion in iron transport by the periplasmic iron-binding protein from Haemophilus influenzae.
Khan AG, Shouldice SR, Tari LW, Schryvers AB
Biochem J 403 43-8 (2007)
PMID: 17147516

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-utilization periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 33.77 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P35755 (Residues: 24-332; Coverage: 100%)
Gene names: HI_0097, fbp, fbpA, hitA
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 105.612Å b: 75.429Å c: 33.436Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.259
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Molecular strategies of microbial iron assimilation: from high-affinity complexes to cofactor assembly systems.
Miethke M. (2013)
6 other articles

1 mention without citation

The role of the synergistic phosphate anion in iron transport by the periplasmic iron-binding protein from Haemophilus influenzae.
Khan et al. (2007)