› 2o6v

X-ray diffraction
2.2Å resolution

Crystal structure and solution NMR studies of Lys48-linked tetraubiquitin at neutral pH

Released:
DOI: 10.2210/pdb2o6v/pdb
PDB entry 2o6v coloured by chain, front view.
PDB entry 2o6v coloured by chain, side view.
PDB entry 2o6v coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure and solution NMR studies of Lys48-linked tetraubiquitin at neutral pH.
Eddins MJ, Varadan R, Fushman D, Pickart CM, Wolberger C
J Mol Biol 367 204-11 (2007)
PMID: 17240395

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143329 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Ubiquitin Chains: B, F
Molecule details ›
Chains: B, F
Length: 76 amino acids
Theoretical weight: 8.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Ubiquitin Chains: D, H
Molecule details ›
Chains: D, H
Length: 76 amino acids
Theoretical weight: 8.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand MES 2 x MES
1 modified residue:
Ligand SLZ 2 x SLZ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: C2
Unit cell:
a: 59.1Å b: 77.08Å c: 139.36Å
α: 90° β: 90.32° γ: 90°
R-values:
R R work R free
0.24 0.222 0.262
Expression system: Escherichia coli

Quick links

Citations

31 review citations

The ubiquitin code.
Komander et al. (2012)
30 more

24 mentions without citation

Unraveling the complexity of ubiquitin signaling.
Strieter et al. (2012)
23 more