› 2obj

X-ray diffraction
2.5Å resolution

Crystal structure of human PIM-1 Kinase in complex with inhibitor

Released:
DOI: 10.2210/pdb2obj/pdb
PDB entry 2obj coloured by chain, front view.
PDB entry 2obj coloured by chain, side view.
PDB entry 2obj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification and structure-activity relationships of substituted pyridones as inhibitors of Pim-1 kinase.
Cheney IW, Yan S, Appleby T, Walker H, Vo T, Yao N, Hamatake R, Hong Z, Wu JZ
Bioorg Med Chem Lett 17 1679-83 (2007)
PMID: 17251021

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase pim-1 Chain: A
Molecule details ›
Chain: A
Length: 333 amino acids
Theoretical weight: 37.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11309 (Residues: 1-313; Coverage: 100%)
Gene name: PIM1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand VRV 1 x VRV
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P65
Unit cell:
a: 97.264Å b: 97.264Å c: 80.827Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.205 0.246
Expression system: Escherichia coli

Quick links

Citations

6 review citations

PIM serine/threonine kinases in the pathogenesis and therapy of hematologic malignancies and solid cancers.
Brault et al. (2010)
5 more

16 mentions without citation

Bioactive 2-pyridone-containing heterocycle syntheses using multicomponent reactions.
Hurtado-Rodríguez et al. (2022)
15 more