› 2ov3

X-ray diffraction
2.4Å resolution

Crystal structure of 138-173 ZnuA deletion mutant plus zinc bound

Released:
DOI: 10.2210/pdb2ov3/pdb
PDB entry 2ov3 coloured by chain, front view.
PDB entry 2ov3 coloured by chain, side view.
PDB entry 2ov3 coloured by chain, top view.
Source organism: Synechocystis sp.
Primary publication:
Possible regulatory role for the histidine-rich loop in the zinc transport protein, ZnuA.
Wei B, Randich AM, Bhattacharyya-Pakrasi M, Pakrasi HB, Smith TJ
Biochemistry 46 8734-43 (2007)
PMID: 17616151

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159878 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic binding protein component of an ABC type zinc uptake transporter Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 28.68 KDa
Source organism: Synechocystis sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P73085 (Residues: 47-137, 174-338; Coverage: 76%)
Gene name: zntC
Sequence domains: Zinc-uptake complex component A periplasmic
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2221
Unit cell:
a: 62.329Å b: 76.982Å c: 127.311Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
5 more

1 mention without citation

Functional characterisation of substrate-binding proteins to address nutrient uptake in marine picocyanobacteria.
Ford et al. (2021)