2p2u

X-ray diffraction
2.75Å resolution

Crystal structure of putative host-nuclease inhibitor protein Gam from Desulfovibrio vulgaris

Released:
Source organism: Desulfovibrio vulgaris
Entry authors: Bonanno JB, Rutter M, Bain KT, Zhang F, Romero R, Smith D, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-180791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Host-nuclease inhibitor protein Gam, putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 171 amino acids
Theoretical weight: 19.48 KDa
Source organism: Desulfovibrio vulgaris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72CZ5 (Residues: 2-170; Coverage: 99%)
Gene name: DVU_1136
Sequence domains: Bacteriophage Mu Gam like protein
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21212
Unit cell:
a: 58.03Å b: 93.408Å c: 104.166Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.242 0.29
Expression system: Escherichia coli BL21(DE3)