› 2p3j

X-ray diffraction
1.9Å resolution

Crystal structure of the Arg101Ala mutant protein of Rhesus rotavirus VP8*

Released:
DOI: 10.2210/pdb2p3j/pdb
PDB entry 2p3j coloured by chain, front view.
PDB entry 2p3j coloured by chain, side view.
PDB entry 2p3j coloured by chain, top view.
Source organism: Simian rotavirus A strain RRV
Primary publication:
Effects on sialic acid recognition of amino acid mutations in the carbohydrate-binding cleft of the rotavirus spike protein.
Kraschnefski MJ, Bugarcic A, Fleming FE, Yu X, von Itzstein M, Coulson BS, Blanchard H
Glycobiology 19 194-200 (2009)
PMID: 18974199

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer capsid protein VP8* Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 18.07 KDa
Source organism: Simian rotavirus A strain RRV
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12473 (Residues: 64-224; Coverage: 21%)
Sequence domains: Outer Capsid protein VP4 (Hemagglutinin) Concanavalin-like domain
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand MNA 1 x MNA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P41212
Unit cell:
a: 48.531Å b: 48.531Å c: 131.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

The sweet spot: defining virus-sialic acid interactions.
Stencel-Baerenwald et al. (2014)
5 more

3 mentions without citation

Structural Basis of Glycan Recognition of Rotavirus.
Sun et al. (2021)
2 more