› 2p3o

X-ray diffraction
2.76Å resolution

Crystal Structure of Dengue Methyltransferase in Complex with 7MeGpppA and S-Adenosyl-L-homocysteine

Released:
DOI: 10.2210/pdb2p3o/pdb
PDB entry 2p3o coloured by chain, front view.
PDB entry 2p3o coloured by chain, side view.
PDB entry 2p3o coloured by chain, top view.
Source organism: Dengue virus 2
Primary publication:
Structural and functional analysis of methylation and 5'-RNA sequence requirements of short capped RNAs by the methyltransferase domain of dengue virus NS5.
Egloff MP, Decroly E, Malet H, Selisko B, Benarroch D, Ferron F, Canard B
J Mol Biol 372 723-36 (2007)
PMID: 17686489

Function and Biology Details

Reactions catalysed: 
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-194371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
type II methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 34.4 KDa
Source organism: Dengue virus 2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WLZ5 (Residues: 2495-2787; Coverage: 9%)
Gene name: DENPoly
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

4 bound ligands:
Ligand SO4 9 x SO4
Ligand SAH 1 x SAH
Ligand CIT 1 x CIT
Ligand GTA 1 x GTA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3121
Unit cell:
a: 111.56Å b: 111.56Å c: 56.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.195 0.244
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Organelle-like membrane compartmentalization of positive-strand RNA virus replication factories.
den Boon et al. (2010)
36 more

9 mentions without citation

Structure and functionality in flavivirus NS-proteins: perspectives for drug design.
Bollati et al. (2010)
8 more