› 2p8e

X-ray diffraction
1.82Å resolution

Crystal structure of the serine/threonine phosphatase domain of human PPM1B

Released:
DOI: 10.2210/pdb2p8e/pdb
PDB entry 2p8e coloured by chain, front view.
PDB entry 2p8e coloured by chain, side view.
PDB entry 2p8e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural genomics of protein phosphatases.
Almo SC, Bonanno JB, Sauder JM, Emtage S, Dilorenzo TP, Malashkevich V, Wasserman SR, Swaminathan S, Eswaramoorthy S, Agarwal R, Kumaran D, Madegowda M, Ragumani S, Patskovsky Y, Alvarado J, Ramagopal UA, Faber-Barata J, Chance MR, Sali A, Fiser A, Zhang ZY, Lawrence DS, Burley SK
J Struct Funct Genomics 8 121-40 (2007)
PMID: 18058037

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131096 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein phosphatase 1B Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 34.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75688 (Residues: 2-297; Coverage: 62%)
Gene names: PP2CB, PPM1B
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand OCS 2 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 52.399Å b: 92.315Å c: 118.713Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Aiding and abetting roles of NOX oxidases in cellular transformation.
Block et al. (2012)
23 more

11 mentions without citation

Structural genomics of protein phosphatases.
Almo et al. (2007)
10 more