› 2pjp

X-ray diffraction
2.3Å resolution

Structure of the mRNA-binding domain of elongation factor SelB from E.coli in complex with SECIS RNA

Released:
DOI: 10.2210/pdb2pjp/pdb
PDB entry 2pjp coloured by chain, front view.
PDB entry 2pjp coloured by chain, side view.
PDB entry 2pjp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural insight into a molecular switch in tandem winged-helix motifs from elongation factor SelB.
Soler N, Fourmy D, Yoshizawa S
J Mol Biol 370 728-41 (2007)
PMID: 17537456

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146850 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Selenocysteine-specific elongation factor Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 14.23 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P14081 (Residues: 487-607; Coverage: 20%)
Gene names: JW3563, b3590, fdhA, selB
Sequence domains:
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
SECIS RNA Chain: B
Molecule details ›
Chain: B
Length: 23 nucleotides
Theoretical weight: 7.39 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand MG 17 x MG
Ligand CL 2 x CL
Ligand CA 4 x CA
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2
Unit cell:
a: 103.498Å b: 56.515Å c: 48.414Å
α: 90° β: 95.6° γ: 90°
R-values:
R R work R free
0.204 0.2 0.251
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

3 review citations

From keys to bulldozers: expanding roles for winged helix domains in nucleic-acid-binding proteins.
Harami et al. (2013)
2 more

12 mentions without citation

DARS-RNP and QUASI-RNP: new statistical potentials for protein-RNA docking.
Tuszynska et al. (2011)
11 more