› 2pnt

X-ray diffraction
2.15Å resolution

Crystal structure of the PDZ domain of human GRASP (GRP1) in complex with the C-terminal peptide of the metabotropic glutamate receptor type 1

Released:
DOI: 10.2210/pdb2pnt/pdb
PDB entry 2pnt coloured by chain, front view.
PDB entry 2pnt coloured by chain, side view.
PDB entry 2pnt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unusual binding interactions in PDZ domain crystal structures help explain binding mechanisms.
Elkins JM, Gileadi C, Shrestha L, Phillips C, Wang J, Muniz JR, Doyle DA
Protein Sci 19 731-41 (2010)
PMID: 20120020

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein TAMALIN Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 11.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z6J2 (Residues: 97-192; Coverage: 24%)
Gene names: GRASP, TAMALIN
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6522
Unit cell:
a: 72.288Å b: 72.288Å c: 163.311Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.232
Expression system: Escherichia coli

Quick links

Citations

5 review citations

PDZ Domains as Drug Targets.
Christensen et al. (2019)
4 more

3 mentions without citation

Unusual binding interactions in PDZ domain crystal structures help explain binding mechanisms.
Elkins et al. (2010)
2 more