› 2pq4

Solution NMR

NMR solution structure of NapD in complex with NapA1-35 signal peptide

Released:
DOI: 10.2210/pdb2pq4/pdb
PDB entry 2pq4 coloured by chain, ensemble of 20 models, front view.
PDB entry 2pq4 coloured by chain, ensemble of 20 models, side view.
PDB entry 2pq4 coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli K-12
Entry authors: Minailiuc OM, Ekiel I, Milad M, Montreal-Kingston Bacterial Structural Genomics Initiative (BSGI)

Function and Biology Details

Reaction catalysed: 
2 ferrocytochrome + nitrate + 2 H(+) = 2 ferricytochrome + nitrite
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141887 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone NapD Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 9.76 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A9I5 (Residues: 1-87; Coverage: 100%)
Gene names: JW2195, b2207, napD, yojF
Sequence domains: NapD protein
Periplasmic nitrate reductase Chain: B
Molecule details ›
Chain: B
Length: 35 amino acids
Theoretical weight: 3.43 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P33937 (Residues: 1-35; Coverage: 0%)
Gene names: JW2194, b2206, napA, yojC, yojD, yojE

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: CYANA2.1 TORSION ANGLE DYNAMICS
Expression system: Escherichia coli BL21

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