› 2pr5

X-ray diffraction
1.45Å resolution

Structural Basis for Light-dependent Signaling in the Dimeric LOV Photosensor YtvA (Dark Structure)

Released:
DOI: 10.2210/pdb2pr5/pdb
PDB entry 2pr5 coloured by chain, front view.
PDB entry 2pr5 coloured by chain, side view.
PDB entry 2pr5 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural basis for light-dependent signaling in the dimeric LOV domain of the photosensor YtvA.
Möglich A, Moffat K
J Mol Biol 373 112-26 (2007)
PMID: 17764689

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-128569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Blue-light photoreceptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 15.12 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O34627 (Residues: 20-147; Coverage: 49%)
Gene names: BSU30340, pfyP, ytvA
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand FMN 2 x FMN
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21212
Unit cell:
a: 88.846Å b: 91.634Å c: 34.239Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.224
Expression system: Escherichia coli BL21

Quick links

Citations

29 review citations

Engineered photoreceptors as novel optogenetic tools.
Möglich et al. (2010)
28 more

25 mentions without citation

Structure and signaling mechanism of Per-ARNT-Sim domains.
Möglich et al. (2009)
24 more