› 2psj

X-ray diffraction
1.8Å resolution

Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis

Released:
DOI: 10.2210/pdb2psj/pdb
PDB entry 2psj coloured by chain, front view.
PDB entry 2psj coloured by chain, side view.
PDB entry 2psj coloured by chain, top view.
Source organism: Renilla reniformis
Primary publication:
Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis.
Loening AM, Fenn TD, Gambhir SS
J Mol Biol 374 1017-28 (2007)
PMID: 17980388

Function and Biology Details

Reaction catalysed: 
(1a) coelenterazine h + O(2) = coelenterazine h dioxetanone
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151045 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coelenterazine h 2-monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 319 amino acids
Theoretical weight: 37.13 KDa
Source organism: Renilla reniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P27652 (Residues: 1-311; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand CEI 2 x CEI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 51.329Å b: 74.473Å c: 89.249Å
α: 90° β: 103.45° γ: 90°
R-values:
R R work R free
0.189 0.188 0.216
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
16 more

10 mentions without citation

Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis.
Loening et al. (2007)
9 more