› 2puz

X-ray diffraction
1.83Å resolution

Crystal structure of Imidazolonepropionase from Agrobacterium tumefaciens with bound product N-formimino-L-Glutamate

Released:
DOI: 10.2210/pdb2puz/pdb
PDB entry 2puz coloured by chain, front view.
PDB entry 2puz coloured by chain, side view.
PDB entry 2puz coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Primary publication:
X-ray structure of imidazolonepropionase from Agrobacterium tumefaciens at 1.87 A resolution.
Tyagi R, Kumaran D, Burley SK, Swaminathan S
Proteins 69 652-8 (2007)
PMID: 17640072

Function and Biology Details

Reaction catalysed: 
(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H(2)O = N-formimidoyl-L-glutamate + H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Imidazolonepropionase Chains: A, B
Molecule details ›
Chains: A, B
Length: 419 amino acids
Theoretical weight: 44.45 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U8Z6 (Residues: 1-419; Coverage: 100%)
Gene names: AGR_L_1824, Atu3934, hutI
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand FE 2 x FE
Ligand MG 1 x MG
Ligand CL 1 x CL
Ligand NIG 1 x NIG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2
Unit cell:
a: 140.913Å b: 64.371Å c: 103.876Å
α: 90° β: 112.27° γ: 90°
R-values:
R R work R free
0.198 0.198 0.225
Expression system: Escherichia coli BL21(DE3)

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Citations

4 mentions without citation

Ligands in PSI structures.
Kumar et al. (2010)
3 more