› 2q46

X-ray diffraction
1.8Å resolution

Ensemble refinement of the protein crystal structure of gene product from Arabidopsis thaliana At5g02240

Released:
DOI: 10.2210/pdb2q46/pdb
PDB entry 2q46 coloured by chain, ensemble of 8 models, front view.
PDB entry 2q46 coloured by chain, ensemble of 8 models, side view.
PDB entry 2q46 coloured by chain, ensemble of 8 models, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Ensemble refinement of protein crystal structures: validation and application.
Levin EJ, Kondrashov DA, Wesenberg GE, Phillips GN
Structure 15 1040-52 (2007)
PMID: 17850744

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188226 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein At5g02240 Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 27.09 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94EG6 (Residues: 2-253; Coverage: 100%)
Gene names: At5g02240, T7H20_290
Sequence domains: NAD(P)H-binding
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NAP 2 x NAP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 46.75Å b: 69.799Å c: 83.828Å
α: 90° β: 96.01° γ: 90°
R-values:
R R work R free
0.209 0.209 0.267
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Enzymes in jasmonate biosynthesis - structure, function, regulation.
Schaller et al. (2009)
18 more