› 2q5z

X-ray diffraction
2.3Å resolution

Crystal structure of iMazG from Vibrio DAT 722: Ntag-iMazG (P43212)

Released:
DOI: 10.2210/pdb2q5z/pdb
PDB entry 2q5z coloured by chain, front view.
PDB entry 2q5z coloured by chain, side view.
PDB entry 2q5z coloured by chain, top view.
Source organism: Vibrio sp. DAT722
Primary publication:
A putative house-cleaning enzyme encoded within an integron array: 1.8 A crystal structure defines a new MazG subtype.
Robinson A, Guilfoyle AP, Harrop SJ, Boucher Y, Stokes HW, Curmi PM, Mabbutt BC
Mol Microbiol 66 610-21 (2007)
PMID: 17892463

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NTP pyrophosphohydrolase MazG-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 114 amino acids
Theoretical weight: 13.1 KDa
Source organism: Vibrio sp. DAT722
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2F9Z1 (Residues: 1-94; Coverage: 100%)
Sequence domains: MazG nucleotide pyrophosphohydrolase domain
Structure domains: MazG-like

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 63.333Å b: 63.333Å c: 163.227Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.207
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The function of integron-associated gene cassettes in Vibrio species: the tip of the iceberg.
Rapa et al. (2013)
12 other articles

3 mentions without citation

Structural and functional insights into DR2231 protein, the MazG-like nucleoside triphosphate pyrophosphohydrolase from Deinococcus radiodurans.
Gonçalves et al. (2011)
2 more