› 2q9o

X-ray diffraction
1.3Å resolution

Near-atomic resolution structure of a Melanocarpus albomyces laccase

Released:
DOI: 10.2210/pdb2q9o/pdb
PDB entry 2q9o coloured by chain, front view.
PDB entry 2q9o coloured by chain, side view.
PDB entry 2q9o coloured by chain, top view.
Source organism: Melanocarpus albomyces
Primary publication:
A near atomic resolution structure of a Melanocarpus albomyces laccase.
Hakulinen N, Andberg M, Kallio J, Koivula A, Kruus K, Rouvinen J
J Struct Biol 162 29-39 (2008)
PMID: 18249560

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180696 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Laccase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 559 amino acids
Theoretical weight: 61.86 KDa
Source organism: Melanocarpus albomyces
Expression system: Trichoderma reesei
UniProt:
  • Canonical: Q70KY3 (Residues: 51-609; Coverage: 93%)
Gene name: LAC1
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
6 bound ligands:
Ligand NAG 5 x NAG
Ligand CU 8 x CU
Ligand CL 2 x CL
Ligand SO4 5 x SO4
Ligand OXY 2 x OXY
Ligand GOL 2 x GOL
1 modified residue:
Ligand OHI 2 x OHI

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 173.74Å b: 61.9Å c: 124.21Å
α: 90° β: 96.36° γ: 90°
R-values:
R R work R free
0.167 0.167 0.212
Expression system: Trichoderma reesei

Quick links

Citations

5 review citations

Heterologous laccase production and its role in industrial applications.
Piscitelli et al. (2010)
4 more

9 mentions without citation

The Father, Son and Cholix Toxin: The Third Member of the DT Group Mono-ADP-Ribosyltransferase Toxin Family.
Lugo et al. (2015)
8 more