› 2qaa

X-ray diffraction
1.23Å resolution

Crystal structure of the second tetrahedral intermediates of SGPB at pH 7.3

Released:
DOI: 10.2210/pdb2qaa/pdb
PDB entry 2qaa coloured by chain, front view.
PDB entry 2qaa coloured by chain, side view.
PDB entry 2qaa coloured by chain, top view.
Source organism: Streptomyces griseus
Primary publication:
1.2A-resolution crystal structures reveal the second tetrahedral intermediates of streptogrisin B (SGPB).
Lee TW, James MN
Biochim Biophys Acta 1784 319-34 (2008)
PMID: 18157955

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with trypsin-like specificity.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo 24-mer
Assembly name:
PDBe Complex ID:
PDB-CPX-133486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptogrisin-B Chains: A, B
Molecule details ›
Chains: A, B
Length: 185 amino acids
Theoretical weight: 18.65 KDa
Source organism: Streptomyces griseus
UniProt:
  • Canonical: P00777 (Residues: 115-299; Coverage: 71%)
Gene name: sprB
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

8 bound ligands:
Ligand SO4 5 x SO4
Ligand CL 10 x CL
Ligand TYR 2 x TYR
Ligand LEU 2 x LEU
Ligand EDO 4 x EDO
Ligand EPE 1 x EPE
Ligand GOL 4 x GOL
Ligand ACY 3 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: I23
Unit cell:
a: 142.286Å b: 142.286Å c: 142.286Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.113 0.113 0.127

Quick links

Citations

1 review citation

Surface loops of trypsin-like serine proteases as determinants of function.
Goettig et al. (2019)
3 other articles

2 mentions without citation

Proteolytic activation of both components of the cation stress-responsive Slt pathway in Aspergillus nidulans.
Mellado et al. (2016)
1 more