› 2qb8

X-ray diffraction
1.9Å resolution

Saccharomyces cerevisiae cytosolic exopolyphosphatase, ATP complex

Released:
DOI: 10.2210/pdb2qb8/pdb
PDB entry 2qb8 coloured by chain, front view.
PDB entry 2qb8 coloured by chain, side view.
PDB entry 2qb8 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure of the cytosolic exopolyphosphatase from Saccharomyces cerevisiae reveals the basis for substrate specificity.
Ugochukwu E, Lovering AL, Mather OC, Young TW, White SA
J Mol Biol 371 1007-21 (2007)
PMID: 17599355

Function and Biology Details

Reaction catalysed: 
(Polyphosphate)(n) + H(2)O = (polyphosphate)(n-1) + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 45.11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38698 (Residues: 1-397; Coverage: 100%)
Gene names: PPX1, YHR201C
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 78.134Å b: 82.847Å c: 121.761Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The long and short of it - polyphosphate, PPK and bacterial survival.
Brown et al. (2008)
2 more