› 2qj3

X-ray diffraction
3Å resolution

Mycobacterium tuberculosis FabD

Released:
DOI: 10.2210/pdb2qj3/pdb
PDB entry 2qj3 coloured by chain, front view.
PDB entry 2qj3 coloured by chain, side view.
PDB entry 2qj3 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structure of Mycobacterium tuberculosis mtFabD, a malonyl-CoA:acyl carrier protein transacylase (MCAT).
Ghadbane H, Brown AK, Kremer L, Besra GS, Fütterer K
Acta Crystallogr Sect F Struct Biol Cryst Commun 63 831-5 (2007)
PMID: 17909282

Function and Biology Details

Reaction catalysed: 
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161857 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malonyl CoA-acyl carrier protein transacylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 32.99 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WNG5 (Residues: 1-302; Coverage: 100%)
Gene names: MTCY427.24, Rv2243, fabD
Sequence domains: Acyl transferase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NI 5 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6222
Unit cell:
a: 180.24Å b: 180.24Å c: 95.99Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.224 0.267
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The pup-proteasome system of Mycobacterium tuberculosis.
Samanovic et al. (2013)
3 more

3 mentions without citation

Fatty acid biosynthesis revisited: structure elucidation and metabolic engineering.
Beld et al. (2015)
2 more