› 2qj6

X-ray diffraction
2.5Å resolution

Crystal structure analysis of a 14 repeat C-terminal fragment of toxin TcdA in Clostridium difficile

Released:
DOI: 10.2210/pdb2qj6/pdb
PDB entry 2qj6 coloured by chain, front view.
PDB entry 2qj6 coloured by chain, side view.
PDB entry 2qj6 coloured by chain, top view.
Source organism: Clostridioides difficile 630
Entry authors: Albesa-Jove D, Bertrand T, Carpenter L, Lim J, Brown KA, Fairweather N

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Toxin A Chains: A, B
Molecule details ›
Chains: A, B
Length: 332 amino acids
Theoretical weight: 37.24 KDa
Source organism: Clostridioides difficile 630
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16154 (Residues: 2387-2706; Coverage: 12%)
Gene names: tcdA, toxA
Sequence domains:
Structure domains: Cholin Binding

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 40.05Å b: 199.68Å c: 58.72Å
α: 90° β: 90.01° γ: 90°
R-values:
R R work R free
0.228 0.226 0.273
Expression system: Escherichia coli BL21(DE3)

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