› 2ql2

X-ray diffraction
2.5Å resolution

Crystal Structure of the basic-helix-loop-helix domains of the heterodimer E47/NeuroD1 bound to DNA

Released:
DOI: 10.2210/pdb2ql2/pdb
PDB entry 2ql2 coloured by chain, front view.
PDB entry 2ql2 coloured by chain, side view.
PDB entry 2ql2 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of E47-NeuroD1/beta2 bHLH domain-DNA complex: heterodimer selectivity and DNA recognition.
Longo A, Guanga GP, Rose RB
Biochemistry 47 218-29 (2008)
PMID: 18069799

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-115806 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Transcription factor E2-alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 60 amino acids
Theoretical weight: 7.14 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P15806 (Residues: 547-606; Coverage: 9%)
  • Best match: P15806-2 (Residues: 544-603)
Gene names: Alf2, Me2, Tcf3, Tcfe2a
Sequence domains: Helix-loop-helix DNA-binding domain
Structure domains: Helix-loop-helix DNA-binding domain
Neurogenic differentiation factor 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 60 amino acids
Theoretical weight: 6.99 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60867 (Residues: 102-160; Coverage: 17%)
Gene names: Neurod, Neurod1
Sequence domains: Helix-loop-helix DNA-binding domain
Structure domains: Helix-loop-helix DNA-binding domain
DNA (5'-D(*DTP*DAP*DGP*DGP*DCP*DCP*DAP*DTP*DCP*DTP*DGP*DGP*DTP*DCP*DCP*DT)-3') Chains: E, G
Molecule details ›
Chains: E, G
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
DNA (5'-D(*DAP*DGP*DGP*DAP*DCP*DCP*DAP*DGP*DAP*DTP*DGP*DGP*DCP*DCP*DTP*DA)-3') Chains: F, H
Molecule details ›
Chains: F, H
Length: 16 nucleotides
Theoretical weight: 4.93 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21212
Unit cell:
a: 60.31Å b: 169.54Å c: 52.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.248 0.289
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Retinoic acid signaling and neuronal differentiation.
Janesick et al. (2015)
4 more

33 mentions without citation

Mechanisms of Binding Specificity among bHLH Transcription Factors.
de Martin et al. (2021)
32 more