› 2qo5

X-ray diffraction
1.5Å resolution

Crystal structure of the cysteine 91 threonine mutant of zebrafish liver bile acid-binding protein complexed with cholic acid

Released:
DOI: 10.2210/pdb2qo5/pdb
PDB entry 2qo5 coloured by chain, front view.
PDB entry 2qo5 coloured by chain, side view.
PDB entry 2qo5 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
A single amino acid mutation in zebrafish (Danio rerio) liver bile acid-binding protein can change the stoichiometry of ligand binding.
Capaldi S, Guariento M, Saccomani G, Fessas D, Perduca M, Monaco HL
J Biol Chem 282 31008-18 (2007)
PMID: 17670743

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid-binding protein 10-A, liver basic Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.29 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I8L5 (Residues: 2-126; Coverage: 99%)
Gene names: fabp10, fabp10a, zgc:103719, zgc:92741
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand CHD 2 x CHD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 28.012Å b: 63.276Å c: 35.671Å
α: 90° β: 105.51° γ: 90°
R-values:
R R work R free
0.22 0.219 0.249
Expression system: Escherichia coli

Quick links

Citations

4 review citations

A survey of the year 2007 literature on applications of isothermal titration calorimetry.
Bjelić et al. (2008)
3 more

2 mentions without citation

How Diverse Are the Protein-Bound Conformations of Small-Molecule Drugs and Cofactors?
Friedrich et al. (2018)
1 more