› 2qw8

X-ray diffraction
1.6Å resolution

Structure of Eugenol Synthase from Ocimum basilicum

Released:
DOI: 10.2210/pdb2qw8/pdb
PDB entry 2qw8 coloured by chain, front view.
PDB entry 2qw8 coloured by chain, side view.
PDB entry 2qw8 coloured by chain, top view.
Source organism: Ocimum basilicum
Primary publication:
Structure and reaction mechanism of basil eugenol synthase.
Louie GV, Baiga TJ, Bowman ME, Koeduka T, Taylor JH, Spassova SM, Pichersky E, Noel JP
OpenAccess logo PLoS One 2 e993 (2007)
PMID: 17912370

Function and Biology Details

Reaction catalysed: 
Eugenol + a carboxylate + NADP(+) = a coniferyl ester + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eugenol synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 35.65 KDa
Source organism: Ocimum basilicum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15GI4 (Residues: 1-314; Coverage: 100%)
Gene name: EGS1
Sequence domains: NmrA-like family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NO3 1 x NO3
Ligand NAP 2 x NAP
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 53.799Å b: 85.935Å c: 76.153Å
α: 90° β: 107.27° γ: 90°
R-values:
R R work R free
0.205 0.205 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Phenylpropanoid biosynthesis.
Vogt T. (2010)
2 more

2 mentions without citation

Structure and function of enzymes involved in the biosynthesis of phenylpropanoids.
Ferrer et al. (2008)
1 more