› 2r6k

X-ray diffraction
2.51Å resolution

Crystal structure of an I71V hGSTA1-1 mutant in complex with S-hexylglutathione

Released:
DOI: 10.2210/pdb2r6k/pdb
PDB entry 2r6k coloured by chain, front view.
PDB entry 2r6k coloured by chain, side view.
PDB entry 2r6k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The role of a topologically conserved isoleucine in glutathione transferase structure, stability and function.
Achilonu I, Gildenhuys S, Fisher L, Burke J, Fanucchi S, Sewell BT, Fernandes M, Dirr HW
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 776-80 (2010)
PMID: 20606271

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 25.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08263 (Residues: 1-222; Coverage: 100%)
Gene name: GSTA1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GTX 2 x GTX
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 98.409Å b: 93.577Å c: 50.717Å
α: 90° β: 93.05° γ: 90°
R-values:
R R work R free
0.211 0.206 0.298
Expression system: Escherichia coli BL21(DE3)

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Citations

1 mention without citation

The role of a topologically conserved isoleucine in glutathione transferase structure, stability and function.
Achilonu et al. (2010)