› 2r79

X-ray diffraction
2.4Å resolution

Crystal Structure of a Periplasmic Heme Binding Protein from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb2r79/pdb
PDB entry 2r79 coloured by chain, front view.
PDB entry 2r79 coloured by chain, side view.
PDB entry 2r79 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Holo- and apo-bound structures of bacterial periplasmic heme-binding proteins.
Ho WW, Li H, Eakanunkul S, Tong Y, Wilks A, Guo M, Poulos TL
J Biol Chem 282 35796-802 (2007)
PMID: 17925389

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130632 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 29.59 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O68879 (Residues: 26-297; Coverage: 92%)
Gene name: phuT
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand HEM 1 x HEM
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P6322
Unit cell:
a: 132.913Å b: 132.913Å c: 72.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.193 0.247
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Iron uptake and metabolism in pseudomonads.
Cornelis P. (2010)
9 more

3 mentions without citation

Iron Acquisition in Mycobacterium tuberculosis.
Chao et al. (2019)
2 more