› 2uwb

X-ray diffraction
2Å resolution

Crystal structure of the Nasturtium seedling mutant xyloglucanase isoform NXG1-delta-YNIIG

Released:
DOI: 10.2210/pdb2uwb/pdb
PDB entry 2uwb coloured by chain, front view.
PDB entry 2uwb coloured by chain, side view.
PDB entry 2uwb coloured by chain, top view.
Source organism: Tropaeolum majus
Primary publication:
Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: biological implications for cell wall metabolism.
Baumann MJ, Eklöf JM, Michel G, Kallas AM, Teeri TT, Czjzek M, Brumer H
Plant Cell 19 1947-63 (2007)
PMID: 17557806

Function and Biology Details

Reaction catalysed: 
Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xyloglucan endotransglucosylase/hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 30.45 KDa
Source organism: Tropaeolum majus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q07524 (Residues: 25-295; Coverage: 98%)
Sequence domains:
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6
Unit cell:
a: 153.894Å b: 153.894Å c: 83.819Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.193 0.213
Expression system: Komagataella pastoris

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Citations

11 review citations

Evolution and diversity of plant cell walls: from algae to flowering plants.
Popper et al. (2011)
10 more

1 mention without citation

Enzymes in 3D: Synthesis, remodelling, and hydrolysis of cell wall (1,3;1,4)-β-glucans.
Hrmova et al. (2023)