› 2v0r

X-ray diffraction
2.3Å resolution

crystal structure of a hairpin exchange variant (LTx) of the targeting LINE-1 retrotransposon endonuclease

Released:
DOI: 10.2210/pdb2v0r/pdb
PDB entry 2v0r coloured by chain, front view.
PDB entry 2v0r coloured by chain, side view.
PDB entry 2v0r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Determinants for DNA target structure selectivity of the human LINE-1 retrotransposon endonuclease.
Repanas K, Zingler N, Layer LE, Schumann GG, Perrakis A, Weichenrieder O
OpenAccess logo Nucleic Acids Res 35 4914-26 (2007)
PMID: 17626046

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LINE-1 retrotransposable element ORF2 protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 27.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00370 (Residues: 1-196, 197-238; Coverage: 19%)
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 54.726Å b: 70.142Å c: 130.221Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.272
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Integration site selection by retroviruses and transposable elements in eukaryotes.
Sultana et al. (2017)
2 more

1 mention without citation

Structural dissection of sequence recognition and catalytic mechanism of human LINE-1 endonuclease.
Miller et al. (2021)