› 2v4e

X-ray diffraction
2.4Å resolution

A non-cytotoxic DsRed variant for whole-cell labeling

Released:
DOI: 10.2210/pdb2v4e/pdb
PDB entry 2v4e coloured by chain, front view.
PDB entry 2v4e coloured by chain, side view.
PDB entry 2v4e coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
A noncytotoxic DsRed variant for whole-cell labeling.
Strack RL, Strongin DE, Bhattacharyya D, Tao W, Berman A, Broxmeyer HE, Keenan RJ, Glick BS
Nat Methods 5 955-7 (2008)
PMID: 18953349

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-163259 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
RED FLUORESCENT PROTEIN DRFP583 Chain: A
Molecule details ›
Chain: A
Length: 218 amino acids
Theoretical weight: 25.18 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli str. K-12 substr. DH10B
Structure domains: Green fluorescent protein
RED FLUORESCENT PROTEIN DRFP583 Chain: B
Molecule details ›
Chain: B
Length: 218 amino acids
Theoretical weight: 25.18 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli str. K-12 substr. DH10B
Structure domains: Green fluorescent protein
RED FLUORESCENT PROTEIN DRFP583 Chains: C, D, E, F, G, H
Molecule details ›
Chains: C, D, E, F, G, H
Length: 218 amino acids
Theoretical weight: 25.18 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli str. K-12 substr. DH10B
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand NRQ 8 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21212
Unit cell:
a: 115.526Å b: 122.619Å c: 164.851Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.22 0.271
Expression system: Escherichia coli str. K-12 substr. DH10B

Quick links

Citations

13 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
12 more

2 mentions without citation

A noncytotoxic DsRed variant for whole-cell labeling.
Strack et al. (2008)
1 more