› 2v7y

X-ray diffraction
2.37Å resolution

Crystal structure of the molecular chaperone DnaK from Geobacillus kaustophilus HTA426 in post-ATP hydrolysis state

Released:
DOI: 10.2210/pdb2v7y/pdb
Model geometry
Fit model/data
PDB entry 2v7y coloured by chain, front view.
PDB entry 2v7y coloured by chain, side view.
PDB entry 2v7y coloured by chain, top view.
Source organism: Geobacillus kaustophilus HTA426
Primary publication:
Crystal structures of the 70-kDa heat shock proteins in domain disjoining conformation.
Chang YW, Sun YJ, Wang C, Hsiao CD
J Biol Chem 283 15502-11 (2008)
PMID: 18400763

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177373 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperone protein DnaK Chain: A
Molecule details ›
Chain: A
Length: 509 amino acids
Theoretical weight: 55.13 KDa
Source organism: Geobacillus kaustophilus HTA426
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5KWZ7 (Residues: 1-509; Coverage: 84%)
Gene names: GK2504, dnaK
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand PO4 1 x PO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 52.753Å b: 71.455Å c: 183.864Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

HSPA8/HSC70 chaperone protein: structure, function, and chemical targeting.
Stricher et al. (2013)
9 more

18 mentions without citation

The nucleotide exchange factors of Hsp70 molecular chaperones.
Bracher et al. (2015)
17 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO