› 2via

X-ray diffraction
1.67Å resolution

Crystal structure of S172AbsSHMT L-Serine external aldimine

Released:
DOI: 10.2210/pdb2via/pdb
Model geometry
Fit model/data
PDB entry 2via coloured by chain, front view.
PDB entry 2via coloured by chain, side view.
PDB entry 2via coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Entry authors: Rajaram V, Bhavani BS, Prakash V, Appaji Rao N, Savithri HS, Murthy MRN

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181944 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase Chain: A
Molecule details ›
Chain: A
Length: 405 amino acids
Theoretical weight: 44.26 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7SIB6 (Residues: 1-405; Coverage: 97%)
Gene name: glyA
Sequence domains: Serine hydroxymethyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
3 bound ligands:
Ligand SER 1 x SER
Ligand MPD 2 x MPD
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 60.986Å b: 106.283Å c: 57.076Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.209
Expression system: Escherichia coli BL21

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