› 2vlt

X-ray diffraction
2Å resolution

Crystal structure of barley thioredoxin h isoform 2 in the oxidized state

Released:
DOI: 10.2210/pdb2vlt/pdb
PDB entry 2vlt coloured by chain, front view.
PDB entry 2vlt coloured by chain, side view.
PDB entry 2vlt coloured by chain, top view.
Source organism: Hordeum vulgare subsp. vulgare
Primary publication:
Crystal structures of barley thioredoxin h isoforms HvTrxh1 and HvTrxh2 reveal features involved in protein recognition and possibly in discriminating the isoform specificity.
Maeda K, Hägglund P, Finnie C, Svensson B, Henriksen A
Protein Sci 17 1015-24 (2008)
PMID: 18424513

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 13.18 KDa
Source organism: Hordeum vulgare subsp. vulgare
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7XZK2 (Residues: 1-122; Coverage: 100%)
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 47.256Å b: 38.37Å c: 56.554Å
α: 90° β: 109.77° γ: 90°
R-values:
R R work R free
0.19 0.186 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Barley seed proteomics from spots to structures.
Finnie et al. (2009)
2 more

1 mention without citation

Crystal structures of barley thioredoxin h isoforms HvTrxh1 and HvTrxh2 reveal features involved in protein recognition and possibly in discriminating the isoform specificity.
Maeda et al. (2008)