› 2voy

Electron Microscopy
18Å resolution

CryoEM model of CopA, the copper transporting ATPase from Archaeoglobus fulgidus

Released:
DOI: 10.2210/pdb2voy/pdb
PDB entry 2voy coloured by chain, front view.
PDB entry 2voy coloured by chain, side view.
PDB entry 2voy coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a copper pump suggests a regulatory role for its metal-binding domain.
Wu CC, Rice WJ, Stokes DL
Structure 16 976-85 (2008)
PMID: 18547529
Related structures: EMD-5004

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O + Cu(+)(Side 1) = ADP + phosphate + Cu(+)(Side 2)
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-128170 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
Copper-exporting P-type ATPase Chain: A
Molecule details ›
Chain: A
Length: 80 amino acids
Theoretical weight: 8.8 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32220 (Residues: 71-146; Coverage: 10%)
Gene names: BSU33500, copA, yvgX
Sequence domains: Heavy-metal-associated domain
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: B
Molecule details ›
Chain: B
Length: 42 amino acids
Theoretical weight: 4.83 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P04191 (Residues: 36-77; Coverage: 4%)
Gene name: ATP2A1
Sequence domains: Cation transporter/ATPase, N-terminus
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: C
Molecule details ›
Chain: C
Length: 22 amino acids
Theoretical weight: 2.51 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P04191 (Residues: 967-988; Coverage: 2%)
Gene name: ATP2A1
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: D
Molecule details ›
Chain: D
Length: 23 amino acids
Theoretical weight: 2.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P04191 (Residues: 832-854; Coverage: 2%)
Gene name: ATP2A1
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: E
Molecule details ›
Chain: E
Length: 30 amino acids
Theoretical weight: 3.31 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P04191 (Residues: 86-115; Coverage: 3%)
Gene name: ATP2A1
Probable copper-exporting P-type ATPase Chain: F
Molecule details ›
Chain: F
Length: 113 amino acids
Theoretical weight: 11.83 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29777 (Residues: 214-326; Coverage: 14%)
Gene names: AF_0473, copA, pacS
Sequence domains: E1-E2 ATPase
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: G
Molecule details ›
Chain: G
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P04191 (Residues: 243-278; Coverage: 4%)
Gene name: ATP2A1
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: H
Molecule details ›
Chain: H
Length: 48 amino acids
Theoretical weight: 5.09 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P04191 (Residues: 289-336; Coverage: 5%)
Gene name: ATP2A1
Probable copper-exporting P-type ATPase Chain: I
Molecule details ›
Chain: I
Length: 128 amino acids
Theoretical weight: 13.78 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29777 (Residues: 520-663; Coverage: 16%)
Gene names: AF_0473, copA, pacS
Sequence domains: haloacid dehalogenase-like hydrolase
Probable copper-exporting P-type ATPase Chain: J
Molecule details ›
Chain: J
Length: 118 amino acids
Theoretical weight: 12.71 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29777 (Residues: 432-549; Coverage: 15%)
Gene names: AF_0473, copA, pacS
Sequence domains: haloacid dehalogenase-like hydrolase
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: K
Molecule details ›
Chain: K
Length: 32 amino acids
Theoretical weight: 3.62 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P04191 (Residues: 749-780; Coverage: 3%)
Gene name: ATP2A1
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: L
Molecule details ›
Chain: L
Length: 21 amino acids
Theoretical weight: 2.22 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P04191 (Residues: 789-809; Coverage: 2%)
Gene name: ATP2A1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
Resolution: 18Å
Relevant EMDB volumes: EMD-5004
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Copper metallochaperones.
Robinson et al. (2010)
17 more

4 mentions without citation

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
3 more