› 2vx7

X-ray diffraction
1.8Å resolution

CELLVIBRIO JAPONICUS MANNANASE CJMAN26C MANNOBIOSE-BOUND FORM

Released:
DOI: 10.2210/pdb2vx7/pdb
PDB entry 2vx7 coloured by chain, front view.
PDB entry 2vx7 coloured by chain, side view.
PDB entry 2vx7 coloured by chain, top view.
Source organism: Cellvibrio japonicus
Primary publication:
The Cellvibrio japonicus mannanase CjMan26C displays a unique exo-mode of action that is conferred by subtle changes to the distal region of the active site.
Cartmell A, Topakas E, Ducros VM, Suits MD, Davies GJ, Gilbert HJ
J Biol Chem 283 34403-13 (2008)
PMID: 18799462

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
CELLVIBRIO JAPONICUS MANNANASE CJMAN26C Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 44.33 KDa
Source organism: Cellvibrio japonicus
Expression system: Escherichia coli BL21(DE3)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA
Carbohydrate polymer
1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6122
Unit cell:
a: 84.334Å b: 84.334Å c: 243.295Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.15 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The biochemistry and structural biology of plant cell wall deconstruction.
Gilbert HJ. (2010)
8 more