› 2w2j

X-ray diffraction
1.6Å resolution

Crystal structure of the human carbonic anhydrase related protein VIII

Released:
DOI: 10.2210/pdb2w2j/pdb
PDB entry 2w2j coloured by chain, front view.
PDB entry 2w2j coloured by chain, side view.
PDB entry 2w2j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human carbonic anhydrase-related protein VIII reveals the basis for catalytic silencing.
Picaud SS, Muniz JR, Kramm A, Pilka ES, Kochan G, Oppermann U, Yue WW
Proteins 76 507-11 (2009)
PMID: 19360879

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase-related protein Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35219 (Residues: 1-290; Coverage: 100%)
Gene names: CA8, CALS
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

3 bound ligands:
Ligand CL 3 x CL
Ligand GOL 6 x GOL
Ligand UNX 3 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 44.267Å b: 73.854Å c: 87.908Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Structure, function and applications of carbonic anhydrase isozymes.
Imtaiyaz Hassan et al. (2013)
11 more

3 mentions without citation

Structural Characterization of Carbonic Anhydrase VIII and Effects of Missense Single Nucleotide Variations to Protein Structure and Function.
Sanyanga et al. (2020)
2 more