› 2w6r

X-ray diffraction
2.1Å resolution

Crystal structure of an artificial (ba)8-barrel protein designed from identical half barrels

Released:
DOI: 10.2210/pdb2w6r/pdb
PDB entry 2w6r coloured by chain, front view.
PDB entry 2w6r coloured by chain, side view.
PDB entry 2w6r coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
High-resolution crystal structure of an artificial (betaalpha)(8)-barrel protein designed from identical half-barrels.
Höcker B, Lochner A, Seitz T, Claren J, Sterner R
Biochemistry 48 1145-7 (2009)
PMID: 19166324

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Imidazole glycerol phosphate synthase subunit HisF Chain: A
Molecule details ›
Chain: A
Length: 266 amino acids
Theoretical weight: 29.61 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X0C6 (Residues: 4-245, 123-253; Coverage: 98%)
Gene names: TM_1036, hisF
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 58Å b: 58Å c: 131Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.242 0.285
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.
Currin et al. (2015)
7 more