› 2w7v

X-ray diffraction
2.3Å resolution

periplasmic domain of EpsL from Vibrio parahaemolyticus

Released:
DOI: 10.2210/pdb2w7v/pdb
PDB entry 2w7v coloured by chain, front view.
PDB entry 2w7v coloured by chain, side view.
PDB entry 2w7v coloured by chain, top view.
Source organism: Vibrio parahaemolyticus
Primary publication:
The dimer formed by the periplasmic domain of EpsL from the Type 2 Secretion System of Vibrio parahaemolyticus.
Abendroth J, Kreger AC, Hol WG
J Struct Biol 168 313-22 (2009)
PMID: 19646531

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II secretion system protein L Chains: A, B
Molecule details ›
Chains: A, B
Length: 95 amino acids
Theoretical weight: 10.91 KDa
Source organism: Vibrio parahaemolyticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q87TC9 (Residues: 318-404; Coverage: 22%)
Gene name: VP0141
Sequence domains: GspL periplasmic domain
Structure domains: General secretion pathway protein M, EpsM

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand PO4 2 x PO4
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P62
Unit cell:
a: 87.712Å b: 87.712Å c: 45.589Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.214 0.256
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
4 more

8 mentions without citation

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
7 more