› 2wep

X-ray diffraction
2Å resolution

Yeast Hsp90 N-terminal domain LI-IV mutant with ADP

Released:
DOI: 10.2210/pdb2wep/pdb
PDB entry 2wep coloured by chain, front view.
PDB entry 2wep coloured by chain, side view.
PDB entry 2wep coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis of the radicicol resistance displayed by a fungal hsp90.
Prodromou C, Nuttall JM, Millson SH, Roe SM, Sim TS, Tan D, Workman P, Pearl LH, Piper PW
ACS Chem Biol 4 289-97 (2009)
PMID: 19236053

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent molecular chaperone HSP82 Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 24.86 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P02829 (Residues: 1-220; Coverage: 31%)
Gene names: HSP82, HSP90, YPL240C
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Structure domains: Histidine kinase-like ATPase, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P4322
Unit cell:
a: 74.915Å b: 74.915Å c: 110.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.263
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Targeting the dynamic HSP90 complex in cancer.
Trepel et al. (2010)
7 more

2 mentions without citation

Multidomain structure and correlated dynamics determined by self-consistent FRET networks.
Hellenkamp et al. (2017)
1 more