› 2wjd

X-ray diffraction
2.8Å resolution

Crystal structure of the tyrosine phosphatase Cps4B from Steptococcus pneumoniae TIGR4.

Released:
DOI: 10.2210/pdb2wjd/pdb
PDB entry 2wjd coloured by chain, front view.
PDB entry 2wjd coloured by chain, side view.
PDB entry 2wjd coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
Crystal structures of Wzb of Escherichia coli and CpsB of Streptococcus pneumoniae, representatives of two families of tyrosine phosphatases that regulate capsule assembly.
Hagelueken G, Huang H, Mainprize IL, Whitfield C, Naismith JH
OpenAccess logo J Mol Biol 392 678-88 (2009)
PMID: 19616007

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189552 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase CpsB Chain: A
Molecule details ›
Chain: A
Length: 247 amino acids
Theoretical weight: 28.49 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9AHD4 (Residues: 1-243; Coverage: 100%)
Gene names: SP_0347, cpsB, wzh
Sequence domains: Capsular polysaccharide synthesis, CpsB/CapC
Structure domains: Metal-dependent hydrolases

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand MN 3 x MN
Ligand SM 2 x SM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 88.85Å b: 88.85Å c: 92.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.173 0.232
Expression system: Escherichia coli

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Citations

7 review citations

Capsules of Streptococcus pneumoniae and other bacteria: paradigms for polysaccharide biosynthesis and regulation.
Yother J. (2011)
6 more

2 mentions without citation

Crystal structures of Wzb of Escherichia coli and CpsB of Streptococcus pneumoniae, representatives of two families of tyrosine phosphatases that regulate capsule assembly.
Hagelueken et al. (2009)
1 more