› 2wm9

X-ray diffraction
2.2Å resolution

Structure of the complex between DOCK9 and Cdc42.

Released:
DOI: 10.2210/pdb2wm9/pdb
PDB entry 2wm9 coloured by chain, front view.
PDB entry 2wm9 coloured by chain, side view.
PDB entry 2wm9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Activation of Rho GTPases by DOCK exchange factors is mediated by a nucleotide sensor.
Yang J, Zhang Z, Roe SM, Marshall CJ, Barford D
Science 325 1398-402 (2009)
PMID: 19745154

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158153 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dedicator of cytokinesis protein 9 Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 50.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BZ29 (Residues: 1605-2069; Coverage: 21%)
  • Best match: Q9BZ29-4 (Residues: 1605-2054)
Gene names: DOCK9, KIAA1058, ZIZ1
Sequence domains:
Structure domains:
Cell division control protein 42 homolog Chain: B
Molecule details ›
Chain: B
Length: 190 amino acids
Theoretical weight: 21.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60953 (Residues: 1-188; Coverage: 98%)
Gene name: CDC42
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 88.41Å b: 93.98Å c: 88.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.251
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
18 more

8 mentions without citation

Structural Insights into the Regulation Mechanism of Small GTPases by GEFs.
Toma-Fukai et al. (2019)
7 more