› 2wn7

X-ray diffraction
2.25Å resolution

Structural Basis for Substrate Recognition in the Enzymatic Component of ADP-ribosyltransferase Toxin CDTa from Clostridium difficile

Released:
DOI: 10.2210/pdb2wn7/pdb
PDB entry 2wn7 coloured by chain, front view.
PDB entry 2wn7 coloured by chain, side view.
PDB entry 2wn7 coloured by chain, top view.
Source organism: Clostridioides difficile
Primary publication:
Structural basis for substrate recognition in the enzymatic component of ADP-ribosyltransferase toxin CDTa from Clostridium difficile.
Sundriyal A, Roberts AK, Shone CC, Acharya KR
J Biol Chem 284 28713-9 (2009)
PMID: 19692332

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191885 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP ribosyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 463 amino acids
Theoretical weight: 53.32 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KH42 (Residues: 1-463; Coverage: 100%)
Gene name: cdtA
Sequence domains: ADP-ribosyltransferase exoenzyme
Structure domains: Toxin ADP-ribosyltransferase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand NAD 1 x NAD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 62.057Å b: 46.772Å c: 77.722Å
α: 90° β: 97.73° γ: 90°
R-values:
R R work R free
0.208 0.205 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Clostridium difficile binary toxin CDT: mechanism, epidemiology, and potential clinical importance.
Gerding et al. (2014)
12 more

8 mentions without citation

Clostridium difficile infection.
Smits et al. (2016)
7 more