› 2wp9

X-ray diffraction
2.7Å resolution

Crystal structure of the E. coli succinate:quinone oxidoreductase (SQR) SdhB His207Thr mutant

Released:
DOI: 10.2210/pdb2wp9/pdb
PDB entry 2wp9 coloured by chain, front view.
PDB entry 2wp9 coloured by chain, side view.
PDB entry 2wp9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Perturbation of the quinone-binding site of complex II alters the electronic properties of the proximal [3Fe-4S] iron-sulfur cluster.
Ruprecht J, Iwata S, Rothery RA, Weiner JH, Maklashina E, Cecchini G
J Biol Chem 286 12756-65 (2011)
PMID: 21310949

Function and Biology Details

Reaction catalysed: 
Succinate + a quinone = fumarate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139344 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Succinate dehydrogenase flavoprotein subunit Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 588 amino acids
Theoretical weight: 64.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC41 (Residues: 1-588; Coverage: 100%)
Gene names: JW0713, b0723, sdhA
Sequence domains:
Structure domains:
Succinate dehydrogenase iron-sulfur subunit Chains: B, F, J
Molecule details ›
Chains: B, F, J
Length: 238 amino acids
Theoretical weight: 26.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07014 (Residues: 1-238; Coverage: 100%)
Gene names: JW0714, b0724, sdhB
Sequence domains:
Structure domains:
Succinate dehydrogenase cytochrome b556 subunit Chains: C, G, K
Molecule details ›
Chains: C, G, K
Length: 129 amino acids
Theoretical weight: 14.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69054 (Residues: 1-129; Coverage: 100%)
Gene names: JW0711, b0721, cybA, sdhC
Sequence domains: Succinate dehydrogenase/Fumarate reductase transmembrane subunit
Structure domains: Fumarate reductase/succinate dehydrogenase, transmembrane subunit
Succinate dehydrogenase hydrophobic membrane anchor subunit Chains: D, H, L
Molecule details ›
Chains: D, H, L
Length: 115 amino acids
Theoretical weight: 12.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC44 (Residues: 1-115; Coverage: 100%)
Gene names: JW0712, b0722, sdhD
Sequence domains: Succinate dehydrogenase/Fumarate reductase transmembrane subunit
Structure domains: Fumarate reductase/succinate dehydrogenase, transmembrane subunit

Ligands and Environments


Cofactor: Ligand HEM 3 x HEM

Cofactor: Ligand FAD 3 x FAD
6 bound ligands:
Ligand TEO 3 x TEO
Ligand NA 3 x NA
Ligand FES 3 x FES
Ligand SF4 3 x SF4
Ligand F3S 3 x F3S
Ligand CBE 3 x CBE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 119.853Å b: 183.803Å c: 202.777Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.222
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The assembly, regulation and function of the mitochondrial respiratory chain.
Vercellino et al. (2022)
4 more

4 mentions without citation

Mutagenesis and functional studies with succinate dehydrogenase inhibitors in the wheat pathogen Mycosphaerella graminicola.
Scalliet et al. (2012)
3 more