› 2wq8

X-ray diffraction
2.19Å resolution

Glycan labelling using engineered variants of galactose oxidase obtained by directed evolution

Released:
DOI: 10.2210/pdb2wq8/pdb
Model geometry
Fit model/data
PDB entry 2wq8 coloured by chain, front view.
PDB entry 2wq8 coloured by chain, side view.
PDB entry 2wq8 coloured by chain, top view.
Source organism: Fusarium graminearum
Primary publication:
Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution.
Rannes JB, Ioannou A, Willies SC, Grogan G, Behrens C, Flitsch SL, Turner NJ
J Am Chem Soc 133 8436-9 (2011)
PMID: 21526835

Function and Biology Details

Reaction catalysed: 
D-galactose + O(2) = D-galacto-hexodialdose + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose oxidase Chain: A
Molecule details ›
Chain: A
Length: 661 amino acids
Theoretical weight: 71.19 KDa
Source organism: Fusarium graminearum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CS93 (Residues: 42-680; Coverage: 97%)
Gene name: GAOA
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CU 1 x CU
Ligand CA 1 x CA
Ligand ACY 2 x ACY
Ligand EDO 9 x EDO
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6522
Unit cell:
a: 88.464Å b: 88.464Å c: 410.846Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.182 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Design strategies for bioorthogonal smart probes.
Shieh et al. (2014)
8 more

1 mention without citation

Hematin and CuII control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversion.
Chen et al. (2023)

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO