› 2wra

X-ray diffraction
1.1Å resolution

BclA lectin from Burkholderia cenocepacia complexed with aMan1(aMan1- 6)-3Man trisaccharide

Released:
DOI: 10.2210/pdb2wra/pdb
PDB entry 2wra coloured by chain, front view.
PDB entry 2wra coloured by chain, side view.
PDB entry 2wra coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Primary publication:
Structural basis of the affinity for oligomannosides and analogs displayed by BC2L-A, a Burkholderia cenocepacia soluble lectin.
Lameignere E, Shiao TC, Roy R, Wimmerova M, Dubreuil F, Varrot A, Imberty A
Glycobiology 20 87-98 (2010)
PMID: 19770128

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110046 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Calcium-mediated lectin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 13.78 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B4EH87 (Residues: 2-129; Coverage: 99%)
Gene name: BCAM0186
Sequence domains: Fucose-binding lectin II (PA-IIL)
Structure domains: Calcium-mediated lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
2 bound ligands:
Ligand CA 2 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 66.296Å b: 66.296Å c: 46.159Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.145 0.144 0.173
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Multivalent glycoconjugates as anti-pathogenic agents.
Bernardi et al. (2013)
5 more

2 mentions without citation

An Algorithm for Protein Helix Assignment Using Helix Geometry.
Cao et al. (2015)
1 more