› 2wtz

X-ray diffraction
3Å resolution

MurE ligase of Mycobacterium Tuberculosis

Released:
DOI: 10.2210/pdb2wtz/pdb
PDB entry 2wtz coloured by chain, front view.
PDB entry 2wtz coloured by chain, side view.
PDB entry 2wtz coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
ATP-dependent MurE ligase in Mycobacterium tuberculosis: biochemical and structural characterisation.
Basavannacharya C, Robertson G, Munshi T, Keep NH, Bhakta S
Tuberculosis (Edinb) 90 16-24 (2010)
PMID: 19945347

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161699 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 535 amino acids
Theoretical weight: 55.45 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WJL3 (Residues: 1-535; Coverage: 100%)
Gene names: MTCY270.10, Rv2158c, murE
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand UAG 4 x UAG
Ligand MG 4 x MG
1 modified residue:
Ligand KCX 4 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 76.92Å b: 79.8Å c: 82.92Å
α: 111.09° β: 92.16° γ: 93.98°
R-values:
R R work R free
0.195 0.193 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Structural perspective of peptidoglycan biosynthesis and assembly.
Lovering et al. (2012)
4 more

7 mentions without citation

Structural and functional features of enzymes of Mycobacterium tuberculosis peptidoglycan biosynthesis as targets for drug development.
Moraes et al. (2015)
6 more