› 2wua

X-ray diffraction
1.8Å resolution

Structure of the peroxisomal 3-ketoacyl-CoA thiolase from Sunflower

Released:
DOI: 10.2210/pdb2wua/pdb
PDB entry 2wua coloured by chain, front view.
PDB entry 2wua coloured by chain, side view.
PDB entry 2wua coloured by chain, top view.
Source organism: Helianthus annuus
Primary publication:
Peroxisomal plant 3-ketoacyl-CoA thiolase structure and activity are regulated by a sensitive redox switch.
Pye VE, Christensen CE, Dyer JH, Arent S, Henriksen A
J Biol Chem 285 24078-88 (2010)
PMID: 20463027

Function and Biology Details

Reaction catalysed: 
(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180484 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
acetyl-CoA C-acyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 440 amino acids
Theoretical weight: 46.03 KDa
Source organism: Helianthus annuus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6W6X6 (Residues: 17-449; Coverage: 96%)
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 78.596Å b: 100.692Å c: 105.296Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.216
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

The peroxisome: an update on mysteries.
Islinger et al. (2012)
3 more

2 mentions without citation

Peroxisomal plant 3-ketoacyl-CoA thiolase structure and activity are regulated by a sensitive redox switch.
Pye et al. (2010)
1 more