› 2wvd

X-ray diffraction
2.65Å resolution

Structural and mechanistic insights into Helicobacter pylori NikR function

Released:
DOI: 10.2210/pdb2wvd/pdb
PDB entry 2wvd coloured by chain, front view.
PDB entry 2wvd coloured by chain, side view.
PDB entry 2wvd coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Structural and mechanistic insights into Helicobacter pylori NikR activation.
Bahlawane C, Dian C, Muller C, Round A, Fauquant C, Schauer K, de Reuse H, Terradot L, Michaud-Soret I
OpenAccess logo Nucleic Acids Res 38 3106-18 (2010)
PMID: 20089510

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative nickel-responsive regulator Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 148 amino acids
Theoretical weight: 17.15 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O25896 (Residues: 1-148; Coverage: 100%)
Gene name: HP_1338
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 49.97Å b: 116.98Å c: 123.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
8 more

1 mention without citation

Structural and mechanistic insights into Helicobacter pylori NikR activation.
Bahlawane et al. (2010)